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Two-dimensional TIRF-SIM-traction force microscopy (2D TIRF-SIM-TFM), Nat Commun, 12 Apr 2021

发布时间:2021年04月12日

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Nature Communications, 12 April, 2021, DOI:888真人百家乐赌博

Two-dimensional TIRF-SIM-traction force microscopy (2D TIRF-SIM-TFM)

Liliana Barbieri, Huw Colin-York, Kseniya Korobchevskaya, Di Li, Deanna L. Wolfson, Narain Karedla, Falk Schneider, Balpreet S. Ahluwalia, Tore Seternes, Roy A. Dalmo, Michael L. Dustin, Dong Li & Marco Fritzsche

Abstract

Quantifying small, rapidly evolving forces generated by cells is a major challenge for the understanding of biomechanics and mechanobiology in health and disease. Traction force microscopy remains one of the most broadly applied force probing technologies but typically restricts itself to slow events over seconds and micron-scale displacements. Here, we improve >2-fold spatially and >10-fold temporally the resolution of planar cellular force probing compared to its related conventional modalities by combining fast two-dimensional total internal reflection fluorescence super-resolution structured illumination microscopy and traction force microscopy. This live-cell 2D TIRF-SIM-TFM methodology offers a combination of spatio-temporal resolution enhancement relevant to forces on the nano- and sub-second scales, opening up new aspects of mechanobiology to analysis.

文章链接:http://www.357.11XPJPJ.COM/articles/s41467-021-22377-9

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